WebThe Phospho-ERK (Thr202/Tyr204) assay measures ERK when phosphorylated at Thr202/Tyr204. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. … WebMar 11, 2024 · We sought to develop a homogeneous cellular assay to interrogate phosphorylation of ERK1 after in vitro treatment of cells using numerous inhibitors of the ERK-MAPK pathway. Fig. 1 .A illustrates the RAS/RAF/MEK/ERK pathway downstream of epidermal growth factor receptor (EGFR) activation, where EGFR activates son of …
Requirement for ERK Activation in Cisplatin-induced Apoptosis*
Web48-619MAG Millipore MILLIPLEX MAP Total/Phospho Erk - Cell Signaling Multiplex Assay Download Zoom Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate sa More>> WebERK phosphorylation was detectable as early as 1 min after agonist addition, with a distinct peak at 3–5 min. Optimization of the cell seeding densities showed that 25,000 cells per well have... crystal reports 2016 windows 11
p-ERK 1/2 (Thr202/Tyr204) Assay Kit Manual
WebSep 26, 2024 · The predominant experimental method for measuring ERK phosphorylation in cultured cells and other biological samples is Western blotting, in which target proteins are separated from prepared cell lysates by SDS-PAGE, transferred onto a polymer sheet, followed by detection with protein-specific antibodies ( Mahmood and Yang, 2012 ). Websignals from each assay well to easily normalize the assay. Here, we demonstrate the benefit and utility of normalizing assay signal of phosphorylated protein levels to total protein levels in two different cellular models: ERK 1/2 phosphorylation in human melanoma A375 cells and AKT 1/2/3 phosphorylation in mouse myoblast C2C12 cells. WebOct 15, 2004 · The assay uses infrared-labeled secondary antibodies to detect phospho-ERK, and the signals in the wells of the microtiter plate are quantified by a LI-COR infrared scanner. V (max), EC (50), and functional K (i) values of various D2 and D3 agonists and antagonists determined in this assay are similar to those in the literature. dying hard meaning