Megashortscripttm t7 转录试剂盒

Author: qouk

August undefined, 2024

WebMEGAscript™ T7 试剂盒是一种超高得率体外转录试剂盒。通过改变典型的转录反应条件，可以有效利用极高的核苷酸浓度，从而获得较高的得率。 T7 酶混合物和 10X 反应缓 … Web规格： 50次. 品牌：国产. 货号： PC1380-50. 货号:PC1380. sgRNA体外转录试剂盒采用T7 RNA聚合酶复合物高效转录sgRNA。. 试剂盒中的反应体系根据sgRNA特点进行优化,每个反应可在4小时内获得多达50 μg的sgRNA。. sgRNA纯化后可用Cas9蛋白体外切割及细胞注射 …

SCARA3基因敲除仓鼠模型的构建方法_2

Web5 feb. 2024 · MEGAshortscript TM T7 Transcription Kit 公司名称: Thermo Fisher Scientific 产品编号: AM1354 Similar Protocol Detection of Individual RNA in Fixed Cells and … Web28 mei 2024 · [0087] (1) (以下均在RNA超净台内完成)使用MEGAshortscriptTM T7转录试剂盒，配体外转录反应体系 [0088] [0089] 混匀，37℃反应4h。 [0090] (2)加入0.5μL TURBO DNase，混匀，37℃反应15min。 [0091] (3)将体系转移至0.6mL小管，加入125μL RNase-free ddH2O和15μL Ammonium Acetate Stop Solution，混匀。 [0092] (4)加入等体积下层 … blacklist category

SP6体外转录试剂盒 - GuideChem

WebT7 体外转录试剂盒数量 25 次反应促进剂 T7 运输条件干冰产量 20-30 µg 原始材料 PCR 产物、线性化 DNA 质粒内容与储存 T7 酶混合物、10X 反应缓冲液 2XNTP⁄CAP 溶液 … WebThermo Scientific TranscriptAid T7 高产率转录试剂盒包含可用于 50 次反应（每次 20 µL）的试剂。每次反应可在 2 小时内由 1 µg 模板产生多达 200µg RNA。可扩大反应规 … Web6 jun. 2024 · All RNAs were tagged with the T7 promoter and transcribed in vitro using MEGAshortscriptTM T7 Kit (ThermoFisher Scientific, Inc), and dissolved in high-salt buffer (10 mM Tris 100 mM NaCl, and 1 mM MgCl 2). The same amount of each RNA (5 μM) or water control was heated to 95°C for 10 min and slowly cooled down to room temperature. blacklist cast season 9 episode 3

mMESSAGE mMACHINE™ T7 ULTRA Transcription …

Web23 feb. 2010 · MEGAshortscript™ T7 High Yield Transcription Kit from Applied Biosystems. in vitro transcription is a commonly used method for producing vast copy numbers of … WebT7体外转录试剂盒价格：￥1490/盒产品详情：价格:1490 元/盒品牌:上海联迈生物工程有限公司产地:中国/上海 SP6体外转Ribo-SPIA cDNA扩增试剂盒录试剂盒价格：电议产品详情：价格:电议品牌:上海联迈生物工程有限公司产地:中国/上海体外转录相关产品报价产品标题价格产地公司名称报价日期 pspt18体外转录质粒电议国产/进... VIP 5年上海禾 … blacklist cast season 9 episode 2WebThe HiScribe T7 High Yield RNA Synthesis Kit is an extremely flexible system for in vitro transcription of RNA using T7 RNA Polymerase. The kit allows for synthesis many kinds … ga office of the attorney general

"WebRiboTM RNAmax T7共转录RNA合成试剂盒可利用含有T7启动子序列、AG起始序列和poly (A)尾编码区的DNA模板，在转录过程中掺入合成的帽结构类似物Ribo-Cap1，一步完成成熟mRNA的制备。合成的mRNA能够被有效翻译并能逃避细胞先天免疫反应。我要订购下载说明书此外，本试剂盒还可根据需求对单个或多个核苷酸单体进行替换，制备特殊化学修 … " - Megashortscripttm t7 转录试剂盒

Megashortscripttm t7 转录试剂盒

FASTmiR: an RNA-based sensor for - Oxford Academic

WebThe T7 RiboMAX™ Express Large Scale RNA Production System offers a setup with minimal pipetting steps to increase efficiency and reduce potential errors. In order to … Web29 mrt. 2024 · 除上述 mRNA 体外合成试剂盒外， Jena Bioscienc 还提供一系列相关高质量原料。包括：修饰核苷酸、 T7 RNA 聚合酶、加帽酶、加尾酶等。南京沃博生物科技有限公司致力于为广大科研工作者提供专业高品质实验室公用产品，欢迎拨打服务热线025-57795736,QQ:1076801270或登录网站www.warbio.cn了解更多信息。

Did you know?

WebT7 High Yield Transcription Kit 是优化的体外转录试剂盒，T7 RNA Polymerase 从模板DNA T7 的启动子下游开始合成与DNA 中一条链互补的RNA，简单快速获得大量的RNA 分子，转录时可在底物中加入修饰的核苷酸，制备生物素或染料标记的RNA。本试剂盒以0.5μg 的模板投入量可以产生150 μg-200 μg 的RNA，转录合成的RNA 可用于诸如RNA 结构与功能 … WebT7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at …

Web30 mrt. 2024 · HiScribe™ T7 mRNA 合成试剂盒（含 CleanCap®Reagent AG）优化了 RNA 合成配方，利用三核苷酸帽结构类似物技术，通过一步共转录加帽即可获得带有天然的 Cap-1 结构的 mRNA，且不会影响 RNA 产量。使用含 T7 启动子序列、AG 起始的目标序列和 poly（A）尾编码序列的 DNA 模板，即可转录带有 5’-m7G Cap-1 和 poly（A）尾的 … Web3 sep. 2024 · 3.根据权利要求2所述的scara3基因敲除仓鼠模型的构建方法，其特征在于，在步骤a1)中，利用ncbi网站获得仓鼠scara3基因组信息，选择其第二外显子；利用crispr设计网站选择适合位点，综合评价打靶效率及脱靶概率后，sgrna的靶点序列确定为：gagatcagctggcggtgacag；在步骤a2)中，含有t7启动子、目的基因靶 ...

WebThe T7 RiboMAX™ Express Large Scale RNA Production System offers a setup with minimal pipetting steps to increase efficiency and reduce potential errors. In order to provide this easy setup, a 2X transcription buffer that already includes all four rNTPs is included. The system also provides RQ1 RNase-Free DNase for the degradation of plasmid ... WebThe linearized DNA plasmids and PCR products were recovered and purified and used for generating the Cas9 mRNA and sgRNAs usingamMESSAGEmMACHINET7ultrakit(Ambion,Austin, TX) and MEGAshortscriptTM T7 kit (Ambion, USA) according to recommendations of the manufacturer.

WebMultiplex gene editing with CRISPR Nuclease mRNA. GeneArt CRISPR Nuclease mRNA is a ready-to-transfect wild-type Cas9 mRNA for performing CRISPR/Cas9-mediated …

WebThe mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog, anti-reverse cap analog (ARCA), with a patented high-yield transcription technology to generate RNA transcripts that produce higher protein … ga office of the child advocateWebT7 High Yield Transcription Kit进行了转录反应体系的优化，T7 RNA Polymerase 从模板DNA T7 启动子下游开始合成与DNA中一条链互补的RNA，简单快速获得大量的RNA分子，转 … ga office ems blacklist ccrishttp://www.bjbalb.com/html/Gene-Structure-Function/YTB3052.html ga office of inspector generalhttp://www.bio-thing.com/p3544 blacklist cellphone contractsWebThe mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog, anti-reverse cap analog (ARCA), with a patented high-yield transcription technology to generate RNA transcripts that produce higher protein … blacklist cast season onehttp://www.warbio.cn/news/show-1772.html ga office of the commissioner of insurance