Fitc-conjugated pac-1
Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). WebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor.
Fitc-conjugated pac-1
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WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … WebThe FITC-conjugated antibodies employed in a laboratory for the DFA test may be developed in house or commercially supplied. Some laboratories use a cocktail of monoclonal antibodies raised in mice against prominent antigens (e.g., nucleoprotein or glycoprotein), while others use a polyclonal antirabies antiserum raised in suitable …
Web2. Slowly add FITC to protein with stirring. Cover with foil and stir 2 hours at room temperature. 3. Separate conjugate from free FITC on G-25 column. Collect fractions. 4. Pool fractions containing conjugate. 5. Determine F/P ratio of conjugate spectrophotometrically. 6. Stabilize with 1% bovine serum albumin and 0.1% sodium … WebConjugate: FITC. Order Details. View Available Conjugates. Catalog# & Conjugate Size Price; Alexa Fluor 350 NBP2-62201AF350: Alexa Fluor 405 NBP2-62201AF405 ... (PAC-1) [FITC] and receive a gift card or discount. Submit a Review. Bioinformatics. Gene Symbol: ITGA2B Popular Products ...
WebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … WebData on immunogenicity of adenovirus-vectored vaccine in chronic obstructive pulmonary disease (COPD) patients is limited. Therefore, we aimed to determine the humoral and cellular immune responses after homologous ChAdOx-1 vaccination in subjects with COPD. COPD subjects and age- and sex-matched healthy elderly receiving ChAdOx-1 …
WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.
WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC … flower shops in woodstock new brunswickWebIn Fig. 1a, we used FITC- conjugated anti-human fibrinogen antibody to highlight intercellular reactivity (white dots, red arrow). The round structures (blue arrow) seem to be a series of TTS ... flower shops in woodstock ontarioWebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs. flower shops in woodbridge vaWebJun 1, 2002 · On human platelets, the high affinity conformation of alphaIIbbeta3 is detected by the monoclonal antibody (mAb), PAC-1. However, a reagent with binding specificity to high affinity mouse alphaIIbbeta3 has not been described so far. ... JON/A was conjugated with fluorescein isothiocyanate (JON/A(FITC)) or with R-phycoerythrin (JON/A(PE)) and ... flower shops in woodinville washingtonWebThe antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. ... a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue … flower shops in woodland hills caWeball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... flower shops in worland wyomingWebFITC conjugates of antibodies, lectins, hormones, and growth factors have been used in a variety of immunohistochemical and flow cytometry applications. The protocols have been optimized for antibodies, but may be adapted to other proteins by the end user. flower shops in woolton liverpool