Ea. hy926 cells

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August undefined, 2024

WebJul 27, 2024 · Human umbilical vein endothelial cells (HUVECs, Ea.hy926) were used to observe the cells adhesion to the samples. Firstly, the sterilized samples were put into 24-well culture plates, then the endothelial cells were seeded on the samples under a concentration of 2 × 10 4 cells/mL. After a 24 h incubation period, the samples were … WebOct 25, 2024 · Senescent EA.hy926 cells express a β-gal, which was detected using a Beta Galactosidase (β-gal) Activity Assay kit (BioVison, Milpitas, CA, USA), according to the manufacturer's instructions, based on the formation of a local blue precipitate upon enzyme-mediated cleavage.

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WebApr 13, 2024 · EA.hy926 cells within the logarithmic growth phase were seeded in 96-well plates (1 × 10 4 cells/cm 2) and incubated with different concentrations of phenolic acid (1, 5, or 25 μM) for 1 h. They were then separately co-incubated with TNF-α (10 ng/mL) or without TNF-α for 24 h. ELISA kits (Abcam, Cambridge, UK) were used to determine the ... WebMar 15, 2024 · EA.hy926 cells express very little RNA for caspase-5 and inflammasome sensors, NLRP3 and NLRC4, that are essential for caspase-1 activation . We stimulated … shark® wandvac® power pet cordless hand vac

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WebMar 1, 1996 · EA.hy926 cells were found to be more responsive than HUVECs to thrombin, as a lower concentration of the agonist was necessary to induce maximum monolayer permeability. Responses of HUVECs and EA.hy926 cells to thrombin were consistent with a pathway involving the thrombin receptor. A thrombin mutant in which the active-site … WebDec 30, 2013 · Because EA.hy926 cells do not express the CD138 surface marker, we further investigated CD138 expression on EA.hy926 cells to verify the interaction of the MM-MVs with the EA.hy926 cells. WebA permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells. Life Sci. … shark wandvac self cleaning

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EA.hy 926 - Wikidata

WebPurpose: The aim of the study was to evaluate the effect of piperlongumine (2 and 4 µM) on endothelial EA.hy926 and lung adenocarcinoma A549 cells with regulated expression of profilin-1... WebEA.hy926 endothelial cells were localized at the bottom of the cell insert and the alveolar type II A549 cell on the upper side (Figure 3a). Cells that resemble the round … shark wandvac reviewWebMar 31, 2024 · EA.hy926 cells, transfected with striatin siRNA or controls (untransfected), were treated with estrogen (5 × 10−8 mol/L), and mRNA levels of PTGIS (A) and PTGS1 (B) were determined. Results show no significant difference in the genomic response after 5 hours of estrogen stimulation in the transfected and control cells (*, P < .05 vs vehicle). shark wandvac system best buy

"WebPurpose: The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and … " - Ea. hy926 cells

Ea. hy926 cells

WebJun 21, 2024 · Mechanistically, DOX-treated EA.hy926 cells and HUVECs demonstrated differential expression levels of the BCL-2 family proteins. In conclusion, both EA.hy926 … WebMar 8, 2024 · To determine the contribution of TM to signaling by thrombin on HUVECs, we decided to delete the TM gene in transformed HUVECs (EA.hy926 cells) using the …

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WebJan 10, 2024 · EA.hy926 is a human umbilical vein cell fusion line that is widely used in vascular-related research. LPS causes inflammation in the endangium, which damages the integrity of endothelial cells. WebJun 27, 2013 · EA.hy 926 is a permanent cell line derived by fusing human umbilical vein endothelial cells with cell line A549 [ 23 ]. The culture was maintained according to the protocol provided by the distributor of the cell line …

WebOct 31, 2024 · EA.hy926 endothelial cells were seeded into a 96-well plate at the density of 3 × 104 cells per well with various concentrations of leptin (0, 0.1, 1, 5, 10, or 50 ng/ml). … WebSep 2, 2024 · The EA.hy926 cells, derived from the fusion of human umbilical vein endothelial cells (HUVECs) with a thioguanine-resistant clone of A549, were used as the …

WebIn the present study, we demonstrated that SLT could protect EA.hy926 cells from oxidative stress and cell damage caused by H 2 O 2. Furthermore, our results suggest that this … WebMay 15, 2024 · EA.hy926 endothelial cells were seeded at 9000 cells/cm 2 on 96 well plates pre-coated with Matrigel (5 μL Matrigel mixed in 95 μL DMEM) or without Matrigel (Panel A) or Collagen Type I dissolved in acetic acid at concentrations of 6 and 10 μg/cm 2 (Panel B) and grown for up to 10 days without changing the media. Cell number was …

WebAug 5, 2015 · Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT) assay, while the effect of TDD on the transcription profile of EA.hy926 cells subjected to H2O2-induced oxidative injury was evaluated by microarray analysis. Several signaling pathways, including apoptosis, were significantly …

WebThe human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to … shark wandvac self-empty systemWebApr 7, 2024 · EA.hy926 cells were cultured in 96-well plates and were serum-starved for 12 h in DMEM with 0.2% FBS before the vehicle, ET-1, or plant extract was added for 24 h. The Annexin V FITC Assay Kit (Cayman Chemical) was used and analyzed using Spectra Max Gemini EM at 485/535 nm per manufacturer protocols to determine the percentage of … shark wandvac system 3-in-1WebThis product is no longer available on Biocompare. Product Specs. Item EA.hy926. Company ATCC. Catalog Number CRL-2922. shark wandvac systemWebMay 10, 2024 · Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway … shark wandvac system hsnWebFigure 1 The effect of piperlongumine on the viability of EA.hy926 (A) and A549 (B) cells. Notes: The cells were treated with different concentrations of piperlongumine (1–8 μM) … shark wandvac self empty systemWebEA.hy926 cell line (7), which mai- n tains an extensively differentiated ph-e notype (20), was also found to be r- e fractory to transfection, despite its vigorous growth properties. After opt-i mization of 5 transfection methods for EA.hy926 cells, Teifel et al. (23) found that the best transfection efficiency achieved was 2%. shark wandvac system manualWebEA.hy926 cells were treated with these gene complexes in the culture solution. EA.hy926 cells were treated with pEGFP-ZNF580 as a negative control group, and cells were treated with PEI 10 kDa/pDNA as a positive control group. The expression of GFP in transfected cells was observed under an inverted fluorescent microscope. shark wandvac system cordless vacuum ws630