WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebSep 18, 2024 · National Center for Biotechnology Information
Cisplatin - NCI - National Cancer Institute
WebSep 30, 2024 · Cisplatin was the first platinum-based agent shown to have anti-tumor properties. 2 Cisplatin-based combinations remain first-line therapy for a number of solid tumors including lung, ovarian, and esophageal cancer. 3 It induces DNA damage through DNA cross-linking, thus interfering with the fundamental processes of DNA replication … WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... highlight expiration dates in excel
Construction of cisplatin-resistant Siha cell line. (A) …
WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay. WebApr 9, 2024 · Then cells were treated with cisplatin as indicated concentrations. 24 or 48 h later, 10 μL CCK8 reagent (HY-K0301, MedChemExpress, USA) was added into each well for another 2 h. Then the absorbance was measured at 490 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader (Bio Tek). WebOct 22, 2024 · Cell viability was analyzed by the Cell Counting Kit-8 (CCK8). The relationship of Nrf2 and CD99 was assessed by dual-luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). Bioinformatics analysis was performed to search for the downstream gene of CD99. small numbers synonym